A portrait drawing of an unidentified woman on pink prepared paper. She is shown half length, facing forwards, and holding a flower, perhaps a pink. A medallion at her breast appears to show an ascending figure. Holbein has used a number of different coloured chalks to capture the sitter's hair and headdress and gentle model the contours of her face. Her eyes are painted in blue watercolour and white heightening with thin strokes of black ink for her eyelashes.

The drawing is on a paper bearing a watermark with the coat of arms of Zurich which seems to have been in use after 1536, and which is found on four of the other portrait drawings in the Royal Collection (RCIN 912235, RCIN 912248, RCIN 912207 and RCIN 912243).

The drawing is inscribed black felbet (black velvet) in Holbein's hand. An eighteenth-century inscription (a copy of a mid-sixteenth-century original) at top left identifies the sitter as 'M. Zouch'.

The sitter remains unidentifed, and a number of possible identities have been suggested. These include Mary Zouch, lady-in-waiting to Jane Seymour, Anne Zouche, lady-in-waiting to Anne Boleyn, Margaret, second wife of Richard Zouche, or the Mistress Souch who was gentlewoman to Mary, Duchess of Richmond. The M is more likely to stand for 'Mistress' than 'Mary' and it may be notable that the Duchess of Richmond was shown by Holbein the same frontal pose as the sitter here.