Justus Sustermans was a Flemish painter who worked in Italy. He trained in Antwerp with Willem de Vos and then in Paris with Frans Pourbus the Younger. In 1620, he accompanied the Belgian tapestry weaver, Pieter Fevère, to Florence. Fevère set up the Medici tapestry factory at the request of Cosimo II de' Medici. In 1621, he was commissioned to work on paintings for the celebration of Ferdinand II's accession as grand duke. Sustermans' portrait of Fevère resulted in him being appointed court painter. For the next 60 years, Sustermans painted the portraits of the Medici family and their circle, including portraits of the astronomer Galileo. He also travelled widely, to Vienna, Rome, Modena, Genoa, Parma and Innsbruck.

This miniature is one of the collection of copies of 224 self-portraits by artists in the Uffizi Palace, Florence, that Lord Cowper, the art collector and patron, commissioned Giuseppe Macpherson (1726-1780) to paint. He presented the miniatures to King George III in two batches, in 1773 and 1786. Macpherson followed the original self-portraits quite closely, but copied only the head and shoulders. He inscribed the artists' names on the backs of the miniatures – several differ from those in the modern Uffizi catalogue, notably: Bazzi, Bellini, Campi, Annibale Carracci, Gabbiani, Masaccio, Metsys, Moroni, Pencz, Licinio, Schiavone and Spada. None of the miniatures is signed, apart from Macpherson's own self-portrait, which is inscribed: Giuseppe Macpherson / Autore della serie (Giuseppe Macpherson / Author of the series).Macpherson was born in Florence, the son of Donald Macpherson, a footman in the service of Alexander, 2nd Duke of Gordon. He was a pupil of Pompeo Batoni and painted miniatures and enamel portraits in Italy, France and Germany, finally settling in Florence. A James Macpherson is recorded in London and Paris in 1754 but it is not certain that this is the same person. He was described in 1776 as having a special talent for painting on enamel and as being 'almost the only painter in Europe who possesses this art to perfection'. He had a distinguished client list which included some of the crowned heads and dignitaries of Europe. In 1778, he was invited to add his own self-portrait to the famous painters in the grand duke's collection as it 'would do honour to Florence to enrich the collection with a work which shows that we still have some men of true merit' according to Giuseppe Pelli, director of the Uffizi at the time.