Anne of Denmark is depicted standing in the grounds of Oatlands House, with the palace visible in the distance to the right. She restrains two black-and-white greyhounds on a leash in her left hand, while three others rest at her feet. The greyhounds have her initials, AR, on their collars. A Black groom, wearing the scarlet and gold livery of the House of Oldenburg, holds her horse to the left while looking towards the Queen - his identity is not known. The horse is covered with a rich caparison in the same colours. An owl, symbol of wisdom, perches on a tree above. A scroll inscribed LA MIA GRANDEZZA DAL ECCELSO floats above the Queen's head. This motto ('My greatness is from on high') was a favourite with the Queen.

Queen Anne was an active and enthusiastic participant in the courtly pursuit of hunting. Here wears a green riding habit and a tall-crowned hat with red plumes. Her green low-cut bodice has curved sleeves in the fashionable Spanish style, one of which is worn hanging with the buttons undone. Her bodice is trimmed with multiple layers of lace at the cuffs and neckline and she wears practical leather riding gloves. Her posture, with its jutting elbow and hand on hip, and her frank gaze, are unusually assertive for a portrait of a consort at this date. 

In the same year that this portrait was painted (1617) Inigo Jones completed his alterations at Oatlands for the Queen, including the classical gateway which is visible here. The painting can be interpreted as a tribute to the Queen's love of building and patronage of Inigo Jones as well as a display of her passion for the chase and a deliberate advertisement of the Divine Guidance with which she felt herself inspired.