Anne of Denmark is shown at full-length, standing, resting her left hand on a red velvet covered table and holding an elaborate ostrich feather fan in her right hand. She wears a bodice with long hanging sleeves which is constructed from white silk decorated with tiny floral motifs in red and gold. Her skirt is of the same fabric and is pinned into pleats over a wide wheel farthingale - a fashion of which the Queen was particularly fond. On her standing lace collar she wears the elaborate jewels in the shape of a crowned S, a reference to her mother Sophia of Mecklenburg and a C4 which was a gift in 1611 from her brother Christian IV of Denmark. She also wears an IHS brooch under a Cross. In the background is an elaborate architectural fantasy which does not appear to be by Van Somer, and is not a representation of Inigo Jones's design for the restoration of the West End of St Paul's Cathedral, as later commentators noted, which was not carried out until the reign of Charles I.

This portrait was painted in 1617-18 and was perhaps that described as hanging at Hampton Court in 1649 as a pendant to RCIN 404446. The head is painted on a separate inset piece of canvas. This was apparently a popular image of the Queen. In other versions, reductions and copies she is set against a much simpler background.

The painting appears in Pyne's illustrated 'Royal Residences' of 1819, hanging in The Queen's Gallery at Kensington Palace (RCIN 922155).

Inscribed ANNA D.G.MAG[AE] / BRITANIAE. FRANCI[AE] / HIBERNIAE REG[INA] / AETATIS SV AE 44.