The unknown Lady is shown at bust-length, facing half to the left, and looking at the spectator. She has pale brown hair under a grey turban-like headdress. She wears a yellow-ochre dress with a brown band across the breast and rose-pink sleeves over a white chemise, and a gold and black necklace.

The painting has been little studied, but can be attributed to Puligo through comparison with works such as the Lady with a Music-Book of 1524 (Private Collection) or the Magdalen (Palazzo Pitti). It is probably a late work, painted in the mid 1520s. Around all sides there is a narrow band of unpainted gesso which indicates that the composition is uncut.