A full-length, posthumous portrait of Margaret Tudor, Queen of Scotland (1489-1541). The source was presumably a portrait of the Queen (now lost) which was one of a series of 'Nyne old heads' hanging in the Privy Gallery at Whitehall in the late 1630s, 'Kinge Henrie the 7th daughter who was married to Kinge James the 4th of Scotland. Painted in a Black habbitt with yallow sleeves w^th a little Monkey houlding uppo her hands In a black frame'. The Queen stands in front of a balcony, beyond which is a landscape with a river. On her arm is a marmoset, at this time an animal highly prized for its rarity.

On 4 June 1625 Daniel Mytens was appointed ‘one of our Picture-Drawers of our Chamber in ordinarie’ for life at £20 a year. This picture was hung in the Bear Gallery at Whitehall near Myten's other posthumous portraits of James IV and Mary, Queen of Scots. It was sold to Margaret Thomson on 8 November 1650 and apparently recovered at the Restoration, although it was first recorded 1687 at Somerset House.

Inscribed on the contemporary cartellino: Margarita, vxor. Jacobi quarti. / regis Scotorum, filia henrici / Septimi Anglorum Regis. / aetatis suae 26. (The last line is smaller and paler script.)